A50-Universal Flow Cytometer
Features
- High speed - up to 100k events/sec. (requires optional "full-digital" electronics)
- Volumetric sample system gives absolute count (avoids expensive, problematic counting beads)
- Built in PC with Windows XP & remote assistance option
- Up to nine fluorescence detectors
- One or two light scatter detectors
- A50 model offers up to 3 lasers
Applications
- Immunology (e.g. lymphocyte phenotyping)
- Haematology (e.g. platelet analysis)
- DNA analysis
- Plant ploidy analysis
- Apoptosis & viability
- ...and more
Technical Data
| Installation Requirements: | |
| Size | W32 x H48 x D48cm |
| Weight | Approx. 25kg |
| Power | 100-240VAC, 50/60Hz |
| Cooling | No special requirements |
| Operating Environment | 5 to 35 degrees Centigrade |
| Optics: | |
| Numerical Aperture | NA=0.85 |
| Lasers | A50-Universal: Up to three lasers: 375nm, 405nm, 488nm, 532nm, 635nm or almost any other commercially available wavelength. |
| Optical Filter Blocks | Interchangeable without realignment |
| Light Scatter Optics | One or two scatter detectors Sensitivity: 300nm (latex beads) |
| Fluorescence Optics | Up to 9 colours (channels) with interchangeable filter blocks |
| Optical Detectors | Photomultiplier tubes on all channels |
| Fluidics: | |
| Sample | Volumetric sample injection Adjustable sample aspiration volume, 100-300µl (smaller and larger volumes available on request) Selectable, precise sample flow rate from 1.4 to 100ul/min to suit a wide range of sample dilutions (adjustable during sample acquisition) Optional automatic sampling from 96 well plates or microcentrifuge tubes |
| Sheath | Choice of Closed loop ultra-pure sheath fluid system or refillable sheath tank with level sensor |
| Sample Range | Sample concentrations up to 108 per ml |
| Data Management: | |
| Electronics |
Fast electronics: Up to 100,000 events per second (requires new 'full-digital' electronic option) Threshold software selectable on all channels with 'AND' or 'OR' logic Pulse height and pulse area measurements on all channels Event time stamp for each particle 18 bit data acquisition (5 logarithmic decades) Internal PC with Windows XP operating system Optional external desktop or laptop PC with LAN connection. |
| Software |
Histogram resolution software selectable from 32 to 65535 Display modes: linear and/or logarithmic gain selection for each parameter Unlimited number of histograms and cytograms Amorphous regions of interest (gates) Gating using AND, OR and NOT logic RATIO parameter configurable for any 2 detectors Autocycle module Gain and subtraction settings adjustable during and after acquisition FCS 2.0 compatible files storage, and storage in 11 or 16 bit format Export of statistics from multiple files to Microsoft Excel Export of histogram data in '.csv' format Quick copy to clipboard feature for datagrams Overlaying histograms for easy data comparison. Plus many more features. |
| Data Analysis Automation | Optional Auto-Lymphocyte cluster analysis and gating software |
ISEV, Gothenburg, April 18th-21st 2012
International Society for Extracellular Vesicles.
NEW Light Scatter Size Calibration Kit
Apogee announces a new light scatter size calibration method for submicron particles...
NEW Autosampler
Automatic sampling module for 96 well plates and microcentrifuge tubes.
Carry-le-Rouet, France, November 5th-8th, 2011
5th International Conference on Analysis of Microbial Cells at the Single Cell Level
Messina, Sicily, June 7th-10th, 2011
3rd SItEMICRO course in Microbial Ecology
Cairo, May 22th-25th, 2010
56th Scientific and Standardization Committee (SSC) meeting of the International Society on Thrombosis and Haemostasis (ISTH)
Roma, 18th-21th May 2010
Practical-theoretical course in Microbial Ecology: Detection and Quantification of Microbes in Natural Environments
Troyes en Champagne, 2 et 3 juin 2010
Association Belge de Cytologie Analytique
Oxford, 23rd-24th September 2010
Micro & Nanovesicles in Health & Disease
New Apogee Software
Keep up to date: Contact us now to get release v2-02 for free!
4čme Cours de Rétrovirologie biologique, Dakar (26th Oct - 24th Nov 2009)
This year, new Auto40 features will be presented by Mr Languillat of Inodex, Paris.
8th UK Meeting on Microbiology, Genetics and Molecular Biology
Apogee sponsored this meeting at the University of Nottingham, January 9th 2009.
Dual laser excitation - January 2009
Apogee has delivered its first dual laser flow cytometer with 405nm and 488nm laser.
15th ICASA
Dakar,
Sénégal
3rd-7th December 2008
Apogee exhibited its Auto40 model at ICASA in Dakar
» More ...
Apogee at 3eme Cours de Retrovirologie Biologique
In collaboration with Inodex (France), Apogee exhibited and demonstrated the new 'Auto40' flow cytometer...
New Telephone Numbers
Apogee has a new telephone system. Please note our new phone numbers:
• Manufacturing:
020 8123 6824
• Sales and Technical Support:
020 8123 6831
NEW Low Cost Auto40
Full function, high quality flow cytometer with automated data analysis.
Marine Microbiology:
North-Western Mediterranean Sea Cruise.
New Data Acquisition and Analysis Software (version 1-88)
Ability to overlay data from multiple files.
Dakar, Senegal
Apogee presented the A40-Microbiology to students . . .
Cytometry 2006, Bruges
Thank you to all who visited our booth at 'Cytometry 2006'...
EMBO Workshop, Carlsberg Academy, Copenhagen
"Cell Cycle & Cytoskeletal Elements in Bacteria"
Apogee sponsored this popular and successful meeting and displayed the high sensitivity of the A40-Microbiology ...