'Kairos' cLDEP Particle Sorter
The first ever application of centripetal liquid dielectrophoretic (cLDEP) force to particle sorting: An electric field plucks chosen particles from the stream with Kairos. The method, invented by ApogeeFlow, requires no moving parts and yields far higher speed and precision than piezoelectric methods.
User defined regions of interest on the flow cytometer's histograms allow selection of populations of particles to be sorted based on combinations of any optical parameter. The sort decision is made within 10μs of the laser interrogation and an electric field generated at the moment the particle reaches the sort zone giving high purity, yield and sort frequency. The automated system allows batches of samples to be sorted from and to a 96 well microplate, unattended.
Four laser, microfluidic particle sorter combining:
Kairos, centripetal liquid dielectrophoretic (cLDEP) force to particle sorting
- Electric field plucks chosen particles from the stream
- High stability: force applied close to the laser for excellent timing precision, no moving parts
- Microfluidics avoids droplet sorter breakoff instability and nozzle residue stream disturbance
- High speed: up to 2500 sorts per second from 20μs pulses (analogous to 50kHz droplet frequency)
- Gentle on cells: low pressure system, no droplet charge
ApogeeFlow particle analyser, for extreme small particle applications
- Up to 4 lasers, spatially separated
- 3 light scatter detectors
- 9 fluorescence colours
