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MicroPLUS Flow Cytometer

Extreme Performance

MicroPLUS Models

Performance to measure extracellular vesicles (EVs) and liposomes

  • ApogeeFlow "MicroPLUS" cytometer is optimised for small particle applications without sacrificing the speed (event rates up to 100,000 per second) required for the measurement of small subpopulations which may originate from a particular cell type. ApogeeFlow was the first flow cytometer manufacturer to offer dedicated models optimised for small particle applications with the power to resolve particles scattering as little as a 100nm silica test bead (equivalent to 70nm polystryrene test beads)
  • The latest options include a wide range of laser colours, GxP compliance, light scatter calibration methods
  • 96 well microplate for high throughput and repeatability (walk-away automation)

Performance to study individual virus particles

ApogeeFlow "MicroPLUS" cytometers are used for the development of vaccines and viral vector immunotherapies. Optimised for small particle applications, it has the performance to measure individual virions like influenza, human immunodeficiency virus (HIV), murine leukemia virus, vesiculovirus (e.g. VSIV), rabies virus, herpes simplex virus (HSV), cytomegalovirus (CMV), vaccinia virus.

Performance to study the smallest bacteria

To measure small bacteria and resolve them from background debris by both light scatter and fluorescence requires an optimised cytometer such and the "Micro" or "MicroPLUS". For example, Lawsonia intracellularis (approx. 250nm diameter filamentous bacteria) may be resolved from debris by combination of two high sensitivity light scatter parameters.

Features include:

Our Cytometers are custom built, based on the specifications (lasers & detectors) needed.

  • Up to 4 spatially separated lasers, 12 optical detectors, optional Autosampler for 96 well microplates
  • Novel noise reduction algorithm implemented in an Altera™ FPGA
  • MicroPLUS model tuned for extreme small particle applications (flow nanometry or nanoscale flow cytometry) including extracellular vesicles (EVs), virus applications (flow virometry)
  • MICRO model configure for the study of bacteria
  • Fast PCIe data acquisition electronics delivers up to 100k events per second
  • Support for GxP compliance including automated calibration.

Performance where it counts

MicroPLUS Flow Cytometer Chart

The ApogeeMix beads (Cat# 1493) are intended for instrument performance assessment. The beads range in size from 110nm to 1300nm and include silica beads with a refractive index similar to biological particles.

Don't just take our word for it...

Automation & Flexibility


  • Fully automated cleaning cycles
  • Fully automated start-up and shutdown
  • Autosampler option for labs where throughput and automation are a priority
  • Email alerts notify operator of status changes
  • Volumetric sample system gives absolute count for every sample


  • Fully upgradeable to a 4 laser system with 12 optical detectors plus Autosampler
  • Suitable for a wide range of applications (maximum particle size 100µm)

Microparticles & Microbiology

The MicroPLUS Flow Cytometer is in a class of its own for sub-micron biological particles, and leading the field in extracellular vesicle research.

Extracellular Vesicles
(Microparticles & Exosomes)

  • The MicroPLUS model offers unprecedented sensitivity from two or three light scatter angle ranges
  • Microvesicles are set to provide the next generation of diagnostic assays
    • Blood-based diagnostic tests for cancers
    • Vascular biology & thrombosis research
    • Therapeutic drug delivery
  • Conventional flow cytometers rely on fluorescent probes to measure biological particles smaller than 400nm diameter, so data is poor when the fluorescence is dim and data inconsistent when the brightness of the fluorescent labels varies
  • The MicroPLUS Flow Cytometer's extreme light scatter performance allows particles' light scatter characteristics to be used to discriminate particles of interest

CD61-PE (vertical axis) labelled platelet derived extracellular vesicles shown on a datagram (light scatter on X axis) with regions of interest left over from analysing test beads (both non-fluorescent silica and fluorescent polystyrene). Note that all the CD61 positive vesicles scatter less than 300nm silica bead region ("10 300Si") and that 300nm silica beads scatter less than the sensitivity limit of conventional flow cytometers.


  • Rapid detection of bacteria in wide variety of samples
  • Cell cycle analysis & genetics
  • Cell viability
  • Fluorescent proteins
  • Virology
MicroPLUS Flow Cytometer Chart 2

Lactobacillus and Propioni superimposed on polystyrene bead data demonstrating the importance of refractive index considerations. Most bacteria scatter a bit less than 500nm polystyrene beads. Many bacteria scatter less than 300nm polystyrene beads. The forward scatter detector on conventional flow cytometers is limited to particles which scatter more than 500nm beads so has insufficient sensitivity to be of use. Size & light scatter depend on growth conditions. Microbiology applications require the ability to resolve bacteria by light scatter, whatever the growth conditions, and by more than one light scatter detector.

Flow Virometry

The MicroPLUS model can measure biological particles down to about 100nm diameter by light scatter. This opens up new applications in virology where both light scatter and fluorescence can be measured from the virions.

For further information see:

Josef Vlasak et al.
Use of flow cytometry for characterization of human cytomegalovirus vaccine particles.
Vaccine, March 2016. DOI:10.1016/j.vaccine.2016.03.067

Don't just take our word for it...

...performance where it counts

Platelets & Other Applications


The MicroPLUS Flow Cytometer's high sensitivity is well suited to platelet applications, including:

  • platelet reactivity
  • platelet aggregates
  • circulating activated platelets
  • platelet-derived microparticles
  • calcium flux
  • bacterial contamination

Other Applications

The MicroPLUS Flow Cytometer is suitable for any analytical flow cytometry application where the particles of interest are less than 100µm, including:

  • Rapid detection of bacteria in wide variety of samples
  • Cell cycle analysis & genetics
  • Cell viability
  • Fluorescent proteins
  • Virology

Don't just take our word for it...

Don't just take our word for it...

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