Customer Quotations

Dr Hon Leong

Dr Hon Leong

London Regional Cancer Program Ontario, Canada

"We have access to other flow cytometers but our microvesicle work demands the sub-micron sensitivity and resolution only offered by Apogee's A50-Micro. The technical support from Apogee has always been prompt and effective and the volumetric fluidics and alignment free optics make it easy to use, but above all we chose the A50-Micro because its resolution and sensitivity is outstanding compared to other flow cytometers."

Silvia Montoro-García, PhD

Silvia Montoro-García, PhD

Postdoctoral Research Fellow
1. Centre for Cardiovascular Sciences, University of Birmingham (UK)
2. Department of Cardiology, Hospital Universitario Virgen de la Arrixaca (Murcia, Spain)

"I started my postdoctoral activity at the Centre for Cardiovascular Sciences (University of Birmingham, UK) in June 2010. My main motivations were to identify the biological value of microvesicles in thrombosis and endothelial dysfunction – especially in the context of atherothrombosis. Microparticle (MPs) analysis is very tricky as their small surface poses a problem for discrimination and labelling. My expertise comprises work with different flow cytometers for MPs. Among all the possibilities, we decided to purchase the Apogee A50-Micro due to its remarkable discrimination of small particles and due to its volumetric sampling system which avoids the need for counting beads. We have developed a new flow cytometric methodology representing a highly specific and reproducible approach when used with the A50-Micro high resolution flow cytometer (CV<6%). The technical support from Apogee has allowed the fast and efficient deployment of this technique here in Birmingham, which now represents a core technique that we apply on clinical studies of MPs and which is the focus of collaborations with several groups interested in the field."

Dr Stefano Amalfitano

Dr Stefano Amalfitano

Istituto di Ricerca Sulle Acque (IRSA) del CNR Rome, Italy

"We spent considerable time deciding among all the cytometers on the market. In the end, our choice was Apogee's A50-Micro. After about two years of use, testing and comparing, I can still say it was the right choice, at least for our research field (aquatic microbiology). It is a powerful and versatile machine for routine analysis and the portability and optical stability are added benefits."

University of Trento

Paola Foladori

University of Trento, Department of Civil and Environmental Engineering

Laura Bruni

Autonomous Province of Trento, Agency for the Treatment of Waste Water

"Our 'A40-Micro' flow cytometer gives the performance we need for measuring small bacteria, assessing their viability and absolute count. It has proven very reliable. After many years of use, we appreciate the high sensitivity not only of the fluorescence signals (for example viability assessment), but also of the light scatter signals."

Researcher, CNRS, France

"The Apogee A50-Micro has been designed for microbiological applications. It is thus optimized for very small particles such as marine bacteria and viruses. You can [install] a UV laser, very valuable for the analysis of bacteria thanks to dyes such as DAPI or Hoechst. The software is easy to manipulate and very intuitive."

Dutch researcher.

"To be honest, I am truly impressed by both the performance and the solidity of the A50-Micro. To my experience, detection of 100 nm polystyrene beads, after shipping and without optical alignment, is far from trivial for a flow cytometer. I believe there is even room for subtle improvements to optimize the lower detection limit for scattering, for example by illuminating with a 405 nm laser."

Publications

Publication Authors Title Date
Nature, Scientific Reports 6:36502
DOI: 10.1038/srep36502
Golam Kibria, Erika K. Ramos, Katelyn E. Lee, Sarah Bedoyan, Simo Huang, Ravand Samaeekia, Jaffre J. Athman, Clifford V. Harding, Jan Lötvall, Lyndsay Harris, Cheryl L. Thompson & Huiping Liu A rapid, automated surface protein profiling of single circulating exosomes in human blood 7-Nov-16
PLoS One
2016 Jun 7;11(6):e0156940
DOI: 10.1371/journal.pone.0156940
Jonathan A. Rose, Nicholas Wanner2, Hoi I. Cheong, Kimberly Queisser, Patrick Barrett, Margaret Park, Corrine Hite, Sathyamangla V. Naga Prasad, Serpil Erzurum, Kewal Asosingh Flow Cytometric Quantification of Peripheral Blood Cell β-Adrenergic Receptor Density and Urinary Endothelial Cell-Derived Microparticles in Pulmonary Arterial Hypertension Jun-16
Vaccine
DOI:10.1016/j.vaccine.2016.03.067
Josef Vlasak, Van M. Hoanga, Sianny Christantia, Richard Pelusoa, Fengsheng Lib, Timothy D. Culp.
 
Merck Research Labs
Use of flow cytometry for characterization of human cytomegalovirus vaccine particles. Mar-16
Cytometry Part B
2016; 00B: 000–000.
DOI: 10.1002/cyto.b.21343
Wayne L. Chandler Measurement of Microvesicle Levels in Human Blood Using Flow Cytometry. Feb-16
Oncotarget. 2016 Feb 23;7(8):8839-49
DOI: 10.18632/oncotarget.6983
Colleen N. Biggs1, Khurram M. Siddiqui, Ali A. Al-Zahrani1, Siddika Pardhan, Sabine I. Brett, Qiu Q. Guo, Jun Yang, Philipp Wolf, Nicholas E. Power, Paul N. Durfee, Connor D. MacMillan, Jason L. Townson, Jeffrey C. Brinker, Neil E. Fleshner, Jonathan I. Izawa, Ann F. Chambers, Joseph L. Chin and Hon S. Leong Prostate extracellular vesicles in patient plasma as a liquid biopsy platform for prostate cancer using nanoscale flow cytometry. Jan-16
J Neuropathol Exp Neurol
2015 July ; 74(7): 672–687.
doi:10.1097/NEN.0000000000000207
Yue Yang, PhD1, C. Dirk Keene, MD, PhD1, Elaine R. Peskind, MD2,3, Douglas R. Galasko, MD4, Shu-Ching Hu, MD, PhD5, Eiron Cudaback, PhD1, Angela M. Wilson, MS1, Ge Li, MD, PhD2, Chang-En Yu, PhD6,7, Kathleen S. Montine, PhD1, Jing Zhang, MD, PhD1, Geoffrey S. Baird, MD, PhD1,8, Bradley T. Hyman, MD, PhD9, and Thomas J. Montine, MD, PhD1 Cerebrospinal Fluid Particles in Alzheimer Disease and Parkinson Disease Jul-15
STEM CELLS 2015;33:2748–2761
http://dx.doi.org/ 10.1002/stem.2078
Sylwia Bobis-Wozowicz, Katarzyna Kmiotek, Malgorzata Sekula, Sylwia Kedracka-Krok, Elzbieta Kamycka, Marta Adamiak, Urszula Jankowska, Anna Madetko-Talowska, Michal Sarna, Miroslaw Bik-Multanowski, Jacek Kolcz, Dariusz Boruczkowski, Zbigniew Madeja, Buddhadeb Dawn andEwa K. Zuba-Surma Human Induced Pluripotent Stem Cell-Derived Microvesicles Transmit RNAs and Proteins to Recipient Mature Heart Cells Modulating Cell Fate and Behavior Jun-15
Transfusion 55 (9) MAY 2015
DOI: 10.1111/trf.13128
Anne Black, Annika Pienimaki-Romer, Oliver Kenyon, Gerd Schmitz Platelet-derived extracellular vesicles in plateletpheresis concentrates as a quality control approach: VESICLES IN PLATELET CONCENTRATES May-15
ASM Infection and Immunity
DOI: 10.1128/IAI.00198-15
Yannick R. Brunet, Ahmad Khodr, Laureen Logger, Laurent Aussel, Tâm Mignot, Sylvie Rimsky, Eric Cascalesa. H-NS Silencing of the Salmonella Pathogenicity Island 6-Encoded Type VI Secretion System Limits Salmonella enterica Serovar Typhimurium Interbacterial Killing. Apr-15
J of AOAC International 2015
98.2, 371-378
Miller, Melinda; Ramsaroop, Shawn; Lopez, Chris; Brahmanda, BharathVivione Bioscience RAPID-B® E. coli O157 Test Kit and non-O157 STEC Test Kit Evaluation.Apr-15
J of Extracellular Vesicles 2015
4: 25530
Vendula Pospichalova, Jan Svoboda, Zankruti Dave, Anna Kotrbova, Karol Kaiser, Dobromila Klemova, Ladislav Ilkovics, Ales Hampl, Igor Crha, Eva Jandakova, Lubos Minar, Vit Weinberger and Vitezslav BryjaSimplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer.Mar-15
Biomacromolecules 2015, 16, 807−814
DOI: 10.1021/bm5017139
Yi Ju, Jiwei Cui, Markus Müllner, Tomoya Suma, Ming Hu, and Frank Caruso* Engineering Low-Fouling and pH-Degradable Capsules through the Assembly of Metal-Phenolic Networks Feb-15
European Association of National Metrology Institutes
MetVes Report
Yuana Yuana, Rienk NieuwlandMetrological characterisation of microvesicles from body fluids as non-invasive diagnostic biomarkersAug-14
Journal of Extracellular Vesicles
2014, 3: 23262
http://dx.doi.org/10.3402/jev.v3.23262
Yuana Yuana1, Johannes Levels2, Anita Grootemaat1, Auguste Sturk1 andRienk NieuwlandCo-isolation of extracellular vesicles and high-densitylipoproteins using density gradient ultracentrifugationJul 2014
J Thromb Haemost 2014
DOI:10.1111/jth.12602
van der Pol E, Coumans FAW, Grootemaat AE, Gardiner C, Sargent IL, Harrison P, Sturk A, van Leeuwen TG, NieuwlandParticle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing.Jun-14
Applied Microbiology
DOI:10.1111/jam.12528
S. Tamburini, P. Foladori, G. Ferrentino, S. Spilimbergo and O. JoussonAccurate flow cytometric monitoring of Escherichia coli subpopulations on solid food treated with high pressure carbon dioxide.Apr-14
British Journal of Haematology
DOI:10.1111/bjh.12913
Silvia Montoro-Garcia Eduard Shantsila, Diana Hernandez-Romero, Eva Jover, Mariano Valdes, Francisco Marin and Gregory Y. H. LipSmall-size platelet microparticles trigger platelet and monocyte functionality and modulate thrombogenesis via P-selectin.17-Mar-14
Microbiology (2013)
DOI:10.1099/mic.0.063321-0
Sabrina Tamburini, Annalisa Ballarini, Giovanna Ferrentino, Albertomaria Moro, Paola Foladori, Sara Spilimbergo and Olivier JoussonComparison of quantitative PCR and flow cytometry as cellular viability methods to study bacterial membrane permeabilization following supercritical CO2 treatment. 
ChemSusChem
2013, 6, 433 - 436
Federico Aulenta, Simona Rossetti, Stefano Amalfitano, Mauro Majone, Valter TandoiConductive Magnetite Nanoparticles Accelerate the Microbial Reductive Dechlorination of Trichloroethene by Promoting Interspecies Electron Transfer Processes.10-Feb-13
Atherosclerosis
2013 Jan 29 pii: S0021-9150(13)00064-6 doi: 10.1016/j.atherosclerosis.2013.01.028
Montoro-Garcia S, Shantsila E, Tapp LD, L pez-Cuenca A, Romero AI, Hern ndez-Romero D, Orenes-Pi ero E, Manzano-Fern ndez S, Vald s M, Mar n F, Lip GYSmall-size circulating microparticles in acute coronary syndromes: Relevance to fibrinolytic status, reparative markers and outcomes.29-Jan-13
Thrombosis and Haemostasis
108.2/2012
Silvia Montoro-García; Eduard Shantsila; Esteban Orenes-Piñero; María Luisa Lozano; Gregory Y. H. LipAn innovative flow cytometric approach for small-size platelet microparticles: Influence of calcium.11-May-12
Clinical Vaccine Immunology
10.1128/CVI.00023-12
Prof Francois-Xavier Mbopi-KéouSingle-platform, volumetric, CD45 assisted pan-leucogating Auto40 flow cytometer for absolute and percent CD4 T cell counting in resource-constrained settings: Validation in Cameroon.15-Feb-12
Elsevier Editorial System(tm) for Food Microbiology
Manuscript Number: FM-D-11 00332R2
Dr. Jon G. Wilkes, Ph.D. (NCTR); Randal K Tucker, PhD; James A Montgomery; Willie M Cooper, MS; John B Sutherland, PhD; Dan A Buzatu, PhDReduction of food matrix interference by a combination of sample preparation and multidimensional gating techniques to facilitate rapid, high sensitivity analysis for Escherichia coli serotype O157 by flow cytometry.Nov-11
Thrombosis and Haemostasis
2011 Jun 9(6):1216-24
Chandler, W.; Yeung, Wandy; Tait, JonathanA new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometer.June-11
J Immunol Methods
2011 Sep 30; 372(1-2):7-13.
Dieye TN et alEvaluation of a flow cytometry method for CD4 T cell enumeration based on volumetric primary CD4 gating using thermoresistant reagents.2011
White paper (available from Apogee on request)Randal Owens, Branch Chief Clinical & Biological Science Latrecia Stubbs, ADH Microbiology Batia Borel, ADH, Microbiology (Cooperation with LITMUS RAPID-B, LLC)FERN Level 2 Validation Assessment of the LITMUS RAPID-B E. coli O157 Assay with Nine Product Matrices. Jun-10
Journal of Thrombosis and Haemostasis
8: 1-11
E . SHANTSILA, P . W. KAMPHUISEN* and G. Y. H. LIPCirculating microparticles in cardiovascular disease: Implications for atherogenesis and atherothrombosis.2010
Water Research
44 (2010) 3807-3818
P. Foladori, L. Bruni, S. Tamburini, G. ZiglioDirect quantification of bacterial biomass in influent, effluent and activated sludge of wastewater treatment plants by using flow cytometry.2010
Seminars in Thrombosis & Haemostasis
36, 8, 807-818
Lacroix et alImprovement in Resolution of Particles <500nm on new generation flow cytometers2010
University of Arkansas Medical Sciences (white paper)Litmus Rapid-B, Marie Chow, PhD Professor of Microbiology and ImmunologyTuberculosis Assay Development Overview.Nov-08
Water Research
42 (2008) 3757 - 3766
Paola Foladori, Alberto Quaranta, Giuliano ZiglioUse of silica microspheres having refractive index similar to bacteria for conversion of flow cytometric forward light scatter into biovolume.2008
WATER RESEARCH
41 (2007) 235 - 243
Paola Foladoria, Bruni Laura, Andreottola, Gianni, Ziglio GiulianoEffects of sonication on bacteria viability in wastewater treatment plants evaluated by flow cytometry Fecal indicators, wastewater and activated sludge.2007
PNAS
Vol. 104 no. 8, 2939-2944
Magnus Lundgren and Rolf BernanderGenome-wide transcription map of an archaeal cell cycle.20-Feb-07
www.plosone.orgSebastian Breuert, Thorsten Allers, Gabi Spohn¤, Jo¨rg SoppaRegulated Polyploidy in Halophilic Archaea Methanothermobacter thermautotrophicus.Dec-06
Current Opinion in Microbiology
2005, 8:662-668
Magnus Lundgren and Rolf BernanderArchaeal cell cycle progress.Oct-05
Cytometry Part B (Clinical Cytometry)
68B:43-51 (2005)
Silva Barbesti, Laura Soldini, Guisline Carcelain, Angelique Guignet, Vittorio Colizzi, Barbara Mantelli, Alessandro Corvaglia, Thun Tran-Minh, Fernanda Dorigatti, Brigitte Autran, Adriano Lazzarin, and Alberto BerettaA Simplified Flow Cytometry Method of CD4 And CD8 Cell Counting Based on Thermoresistant Reagents: Implications for Large Scale Monitoring of HIV Infected Patients in Resource Limited Settings.2005
JOURNAL OF BACTERIOLOGYAlan I. Majernı´k, Magnus Lundgren, Paul McDermott, Rolf Bernander and James P. J. ChongDNA Content and Nucleoid Distribution in Methanothermobacter thermautotrophicus.Mar-05
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Nov. 2004, p. 6595-6602
Roberto Borghese,1* Francesca Borsetti,1 Paola Foladori,2 Giuliano Ziglio,2 and Davide Zannoni1Effects of the Metalloid Oxyanion Tellurite (TeO32) on Growth Characteristics of the Phototrophic Bacterium Rhodobacter capsulatus.Nov-04
Water Research
36 (2002) 460-468
Giuliano Ziglio, Gianni Andreottola, Silvia Barbesti, Giorgio Boschetti, Laura Bruni, Paola Foladori, Roberta VillaAssessment of activated sludge viability with flow cytometry.2002
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